Rapid electrophoresis and quantitation of haemoglobins on cellulose acetate.

Rapid cellulose acetate electrophoresis. I. Serum proteins.

A method of electrophoresis utilizing cellulose acetate is described wherein quantitation of serum protein fractions by densitometry is accomplished within 2 hr. of initiation of the procedure. The densitometer was found to have a linear response to amido black dye. Albumin and y-globulin demonstrated equal dye-binding characteristics. Combined with the absence of albumin trailing, this has aff...

The detection of myoglobin in urine and its distinction from normal and variant haemoglobins.

Victims of severe injuries frequently pass haemoglobin, myoglobin, or both proteins in their urine. If the kidneys of such persons are to be considered as donor material for transplantation, a pathology department may be requested to identify which of these pigments is present. If freshly passed urine is available, haemoglobinuria in the absence of myoglobinuria may be rapidly identified by spe...

Separation of haemoglobins on cellulose acetate.

Rapid differentiation of polypeptide chains of hemoglobin by cellulose acetate electrophoresis of hemolylsates.

I DENTIFICATION of the abnormal polypeptide chain of a hemoglobin ( Hb ) variant is often useful in connection with structural analyses and Chernoff and Pettit’ have recently improved on existing methods for this purpose by using starch gel electrophoresis of globin in urea barbital buffer. Izzo2 used this same buffer for electrophoresis of globin on cellulose acetate. Ohba et al.3 have propose...

BRIEF REPORT Rapid Differentiation of Polypeptide Chains of Hemoglobin by Cellulose Acetate Electrophoresis of Hemolylsates

I DENTIFICATION of the abnormal polypeptide chain of a hemoglobin ( Hb ) variant is often useful in connection with structural analyses and Chernoff and Pettit’ have recently improved on existing methods for this purpose by using starch gel electrophoresis of globin in urea barbital buffer. Izzo2 used this same buffer for electrophoresis of globin on cellulose acetate. Ohba et al.3 have propose...